Journal: Cellular immunology

Article Title: Secretion of MIP-1? and MIP-1? by CD8 + T-lymphocytes Correlates with HIV-1 Inhibition Independent of Coreceptor Usage

doi: 10.1016/j.cellimm.2010.09.011

Figure Lengend Snippet: Histone hyperacetylation regulates the suppressive ability of CD8+ T-lymphocytes and expression of cytokines. (A) CD8+ T-lymphocytes were cultured for 16 h in growth media supplemented with 2.0 mM valproic acid (filled bars) or PBS (open bars) as a vehicle control. Subsequently, the CD8+ T-lymphocytes were used in a direct contact noncytolytic CD8 suppression assay. Mean log10 reduction in HIV-1 replication and standard error are shown. Significance was determined with a Student’s t-test (* p < 0.05; ** p < 0.01). (B) RNA was isolated from the valproic acid-treated or control CD8+ T-lymphocytes used in (A), and differences in gene expression of 164 cytokine and cytokine receptor genes were analyzed by real-time RT-PCR Array. Mean fold change in expression of each gene is displayed, with negative fold changes designating down-regulated genes, and positive fold changes signifying genes upregulated in valproic acid-treated cells compared to control cells. Genes within the area shaded in gray were considered unchanged (less than a 3-fold change in expression). Fold down-regulation of genes in JR-HVS (C) and VC18 (D) CD8+ T-lymphocytes after inhibition of histone deacetylation.

Article Snippet: Total RNA was extracted from the remaining treated cells to be analyzed by Real-time Reverse transcriptase-PCR Array (SA Biosciences).

Techniques: Expressing, Cell Culture, Suppression Assay, Isolation, Quantitative RT-PCR, Inhibition